RICERCA ITALIANA     

FIRB simili:

• 1 - Impiego delle tecniche di ingegneria tissutale nello sviluppo di protesi vascolari cellularizzate
• 2 - Cellule staminali adulte pluripotenti per la ricostituzione tissutale in modelli di malattie degenerative
• 3 - Identificazione, purificazione, transdifferenziazione e banking di cellule staminali indirizzate verso fenotipi propri e non propri da utilizzare per riparazione tissutale
• 4 - Approcci combinati di terapia genica nel trattamento del glioblastoma
• 5 - Sviluppo di microsistemi multifunzionali per analisi in diagnostica clinica e nel settore alimentare
• 6 - Studio dell'identità cellulare e dei meccanismi di differenziazione cellulare nell'era della postegnomica utilizzando tre situazioni paradigmatiche: la mammella, l'osso e i linfociti
• 7 - L'ingegneria dei tessuti per la rigenerazione dei tessuti connettivi con particolare riferimento al tessuto osseo
• 8 - Ottimizzazione dell' espansione ex-vivo di cellule staminali e progenitori emopoietici umani
• 9 - BILANCIO OSPITE-TUMORE NELLA TERAPIA MEDICA DELLE NEOPLASIE: RUOLO DI NF-kB
• 10 - Molecole innovative ad attività antiflogistica ed antitumorale prodotte con metodologie chimiche avanzate

Classificazione scientifico-disciplinare

• Area scientifico disciplinare: Scienze chimiche
  • CHIMICA FARMACEUTICA
• Area scientifico disciplinare: Ingegneria industriale e dell'informazione
  • ELETTRONICA

Classificazione brevettuale

• CHEMISTRY; METALLURGY
  • BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY (installation for fermenting manure A01C3/02; preservation of living parts of humans or animals A01N1/02; physical or chemical apparatus in general B01; malting or mashing apparatus C12C1/00; brewing apparatus C12C13/00; fermentation apparatus for wine C12G; apparatus for preparing vinegar C12J1/10)
    • MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
• PHYSICS
  • MEASURING (counting G06M); TESTING
    • INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (separating components of materials in general B01D, B01J, B03, B07; apparatus fully provided for in a single other subclass, see the relevant subclass e.g. B01L; measuring or testing processes other than immunoassay, involving enzymes or micro-organisms C12M, C12Q; investigation of foundation soil in situ E02D1/00; sensing humidity changes for compensating measurements of other variables or for compensating readings of instruments for variations in humidity, see G01D or the relevant subclass for the variable measured; testing or determining the properties of structures G01M; measuring or investigating electric or magnetic properties of materials G01R; systems or methods in general, using reception or emission of radiowaves or other waves and based on propagation effects, e.g. Doppler effect, propagation time, direction of propagation, G01S; determining sensivity, graininess, or density of photographic materials G03C5/02; testing component parts of nuclear reactors G21C17/00; [N: controlling or regulating non-electric variables G05D; measuring degree of ionisation of ionised gases, i.e. plasma H05H1/00A; testing electrographic developer properties G03G15/08H6])

Classificazione geografica

• Regione: Emilia Romagna

Bibliografia

Huang, Y., Wang, X-B, Tame, J. A., and Pethig, R. 1993. "Electrokinetic behavior of colloidal particles in traveling electric fields: studies using yeast cells." J. Phys. D: Appl. Phys. 26: 1528-1535.

Fuhr. G., Arnold, W. M., Hagedorn, R., Muller, T., Benecke, W., Wagner B., and Zimmermann, U. 1992. "Levitation, holding, and rotation of cells within traps made by high-frequency fields." Biochim. Biophys. Acta, 1108: 215-223.

Masuda, S., Washizu, M., and Iwadare, M. 1987. "Separation of small particles suspended in liquid by nonuniform traveling field." IEEE Trans. Ind. Appl., 23: 217-222.

Pethig, R., Huang, Y., Wang, X-B., and Burt, J. P. H. 1992. "Positive and negative dielectrophoretic collection of colloidal particles using interdigitated castellated microelectrodes." J. Phys. D: Appl. Phys., 25: 881-888.

Schnelle, T., Hagedorn R., Fuhr, G., Fiedler, S., and Muller, T. 1993. "Three-dimensional electric field traps for manipulation of cells-calculation and experimental verification." Biochim Biophys. Acta, 1157: 127-140.

Pethig R., "Dielectrophoresis: Using Inhomogeneous AC Electrical Fields to Separate and Manipulate Cells." Critical Rewiews in Biotechnology, 1996. 16: 331-348.

Washizu M., Kurosawa O., Arai I., Suzuki S., and Shimamoto N., "Applications of Electrostatic Stretch-and-positioning of DNA." 1995. IEEE Trans. on Industry Appl., vol.31, n.3, 447-456.

Washizu M., Kurosawa O., "Electrostatic Manipulation of DNA in microfabricated structures." 1990. IEEE Trans. on Industry Appl., Vol.26, n.6, 1165-1172.

Fuhr G., Arnold W. M., Hagedorn R., Muller T., Benecke W., Wagner B., Zimmermann U., 1992. "Levitation, holding, and rotation of cells within traps maded by high-frequency fields." Biochimica et Biophysica Acta, 1108: 215-223.

Wang X-B., Huang Y., Gascoyne R. C. P., and Becker F.F., "Dielectrophoretic manipulation of particles." 1997. IEEE Trans. on Industry Appl. Vol. 33 n.3 660-669.

Huang Y., Wang X-B., Tame J. A., Pethig R., "Electrokinetic behavior of colloidal particles in travelling electric fields: studies using yeast cells." 1993. J. Phys. D: Appl. 26: 1528-1535.

Schnelle T., Hagedorn R., Fhur G., Fidler S., Muller T., "Three-dimensional electric field traps for manipulation of cells-calculation and experimental verification." 1993. Biochimica et Biophysica Acta 1157: 127-140.

Washizu M., "Electrostatic manipulation of biological objects." 1990. Journal of Electrostatics. vol.25 pp. 109-123.

Washizu M., Nanba T., Masuda S., "Handling biological cells using a fluid integrated circuit." 1990. IEEE Trans. on Industry Appl. vol.26, n.2, 352-358.

Masuda S., Washizu M., Kawabata I., "Movement of blood cells in liquid by nonuniform travelin field." 1988. IEEE Trans. on Industry Appl. vol.24, n.2, pp.217-222.

Washizu M., "Precise calculation of Dielectrophoretic force in arbitrary field." 1992. Journal of Electrostatics 29: 177-188.

Washizu M., Jones T. B., Kaler K. V. I. S., "Higher-order dielectrophoretic effects: levitation at a field null." 1993. Biochimica et Biophysica Acta, 1158: 40-46.

Wang X-B., Hughes M. P., Huang Y., Becker F. F., Gascoyne
P. R. C., "Non-uniform spatial distribution of both the magnitude and phase of AC electric fields determine dielectrophoretic forces." 1995.
Biochimica et Biophysica Acta 1243, 185-194.

Jones T. B., Washizu M., "Equilibria and dynamic of DEP-levitated particles: multipolar theory." 1994. Journal of Electrostatics, vol.33, pp. 199-212.

Jones T. B., Kallio G. A., "Dielectrophoretic levitation of spheres and shells." 1979. Joutnal of Electrostatics, vol.6, pp. 207-224.

Wang X-B., Huang Y., Becker F. F., Gascoyne P. R. C., "A unified theory of dielectrophoresis and travelling wave dielectrophoresis." 1994. J.Phys. D: Appl. Phys. vol. 27, pp. 1571-1574.

Bianchi N, Chiarabelli C, Borgatti M, Mischiati C, Fibach E, Gambari R. Accumulation of gamma-globin mRNA and induction of erythroid differentiation after treatment of human leukaemic K562 cells with tallimustine. Br J Haematol. 2001, 113(4):951-61.

Bianchi N, Ongaro F, Chiarabelli C, Gualandi L, Mischiati C, Bergamini P, Gambari R. Induction of erythroid differentiation of human K562 cells by cisplatin analogs. Biochem Pharmacol. 2000; 60(1):31-40.

Bianchi N, Osti F, Rutigliano C, Corradini FG, Borsetti E, Tomassetti M, Mischiati C, Feriotto G, Gambari R. The DNA-binding drugs mithramycin and chromomycin are powerful inducers of erythroid differentiation of human K562 cells. Br J Haematol. 1999;104(2):258-65.

Bianchi N, Rutigliano C, Tomassetti M, Feriotto G, Zorzato F, Gambari R. Biosensor technology and surface plasmon resonance for real-time detection of HIV-1 genomic sequences amplified by polymerase chain reaction. Clin Diagn Virol. 1997; 8(3):199-208.

Bianchi N, Rutigliano C, Passadore M, Tomassetti M, Pippo L, Mischiati C, Feriotto G, Gambari R. Targeting of the HIV-1 long terminal repeat with chromomycin potentiates the inhibitory effects of a triplex-forming oligonucleotide on Sp1-DNA interactions and in vitro transcription. Biochem J. 1997; 326 ( Pt 3):919-27.

Davies J. Surface Plasmon resonance - The technique and its applications to biomaterial processes. Nanobiology 1994; 3: 5-16.

Feriotto G, Lucci M, Bianchi N, Mischiati C, Gambari R. Detection of the deltaF508 (F508del) mutation of the cystic fibrosis gene by surface plasmon resonance and biosensor technology.
Hum Mutat. 1999;13(5):390-400.

Feriotto G, Ferlini A, Ravani A, Calzolari E, Mischiati C, Bianchi N, Gambari R. Biosensor technology for real-time detection of the cystic fibrosis W1282X mutation in CFTR. Hum Mutat. 2001;18(1):70-81.

Gambari R, Feriotto G, Rutigliano C, Bianchi N, Mischiati C. Biospecific interaction analysis (BIA) of low-molecular weight DNA-binding drugs. J Pharmacol Exp Ther. 2000; 294(1):370-

Gambari R. Biospecific Interaction Analysis: a tool for drug discovery and development. Am.J.Pharmacogenomics, 1, 119,135, 2001.

Gambari R. Peptide-nucleic acids (PNAs): a tool for the development of gene expression modifiers. Curr Pharm Des. 2001, 7(17):1839-62.

Malmqvist M. Biospecific interactions analysis using biosensor technology. Nature 1993; 361:186-7.

Mischiati C, Feriotto G, Borgatti M, Giacomini P, Gambari R. Characterization of a major histocompatibility complex class II X-box-binding protein enhancing tat-induced transcription directed by the human immunodeficiency virus type 1 long terminal repeat. J Virol. 2000, 74(19):8989-9001.

Mischiati C, Borgatti M, Bianchi N, Rutigliano C, Tomassetti M, Feriotto G, Gambari R.
Interaction of the human NF-kappaB p52 transcription factor with DNA-PNA hybrids mimicking the J Biol Chem. 1999;274(46):33114-22.

Myszka D.G, Rich R.L. Implementating surface plasmon resonance biosensors in drug discovery. Pharmaceutical Sciences & Technology Today 2000; 9: 3310-7.

Nastruzzi C, Cortesi R, Esposito E, Gambari R, Borgatti M, Bianchi N, Feriotto G, Mischiati C.
Liposomes as carriers for DNA-PNA hybrids. J Control Release. 2000; 68(2):237-49.

Osborne J, Harrison P, Butcher R et al. Novel super-high affinity sheep monoclonal antibodies against CEA bind colon and lung adenocarcinoma. Hybridoma 1999; 18(2):183-91.

Osti F, Corradini FG, Hanau S, Matteuzzi M, Gambari R. Human leukemia K562 cells: induction to erythroid differentiation by guanine, guanosine and guanine nucleotides. Haematologica. 1997; 82(4):395-401.

Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, and Arnheim N (1985). Enzymatic amplification of alpha-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350-1354.

Rutigliano C, Bianchi N, Tomassetti M, Pippo L, Mischiati C, Feriotto G, Gambari R. Surface plasmon resonance for real-time monitoring of molecular interactions between a triple helix forming oligonucleotide and the Sp1 binding sites of human Ha-ras promoter: effects of the DNA-binding drug chromomycin. Int J Oncol. 1998; 12(2):337-43.

Parole Chiave

biochip; microelettronica; bioseparazione; diagnostica molecolare; veicolazione di farmaci