RICERCA ITALIANA     

FIRB simili:

• 1 - Identificazione, purificazione, transdifferenziazione e banking di cellule staminali indirizzate verso fenotipi propri e non propri da utilizzare per riparazione tissutale
• 2 - L'ingegneria dei tessuti per la rigenerazione dei tessuti connettivi con particolare riferimento al tessuto osseo
• 3 - Cellule staminali adulte pluripotenti per la ricostituzione tissutale in modelli di malattie degenerative
• 4 - Approcci combinati di terapia genica nel trattamento del glioblastoma
• 5 - Ottimizzazione dell' espansione ex-vivo di cellule staminali e progenitori emopoietici umani
• 6 - ESPANSIONE E DIFFERENZIAZIONE IN VITRO DI CELLULE STAMINALI CORDONALI E DA LIQUIDO AMNIOTICO DA UTILIZZARE NELL'INGEGNERIA TESSUTALE
• 7 - Studio dell'identità cellulare e dei meccanismi di differenziazione cellulare nell'era della postegnomica utilizzando tre situazioni paradigmatiche: la mammella, l'osso e i linfociti
• 8 - Nuovi strumenti biotecnologici e post-genomici utilizzabili per la rivascolarizzazione dei tessuti nelle malattie ischemiche.
• 9 - Sviluppo di un Lab-on-a-chip basato su tecnologia microelettronica e sua validazione biotecnologica
• 10 - INGEGNERIA TISSUTALE: SVILUPPO DI NUOVE STRATEGIE PER LA RIGENERAZIONE DEL PARODONTO E DELL'OSSO ALVEOLARE MEDIANTE CELLULE MESENCHIMALI DI DERIVAZIONE EMATOPOIETICA NELL'ANIMALE E NELL'UOMO. VALUTAZIONE DEGLI EFFETTI SISTEMICI.

Classificazione scientifico-disciplinare

• Area scientifico disciplinare: Scienze biologiche
  • ISTOLOGIA
• Area scientifico disciplinare: Scienze mediche
  • PATOLOGIA GENERALE
• Area scientifico disciplinare: Ingegneria industriale e dell'informazione
  • BIOINGEGNERIA INDUSTRIALE

Classificazione brevettuale

• CHEMISTRY; METALLURGY
  • BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY (installation for fermenting manure A01C3/02; preservation of living parts of humans or animals A01N1/02; physical or chemical apparatus in general B01; malting or mashing apparatus C12C1/00; brewing apparatus C12C13/00; fermentation apparatus for wine C12G; apparatus for preparing vinegar C12J1/10)
• HUMAN NECESSITIES
  • MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agent employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toilet purposes A61K; preparation of ozone C01B13/10)

Classificazione geografica

• Regione: Toscana

Bibliografia

1) Abbot WM, Cullow A, Moore W, et al. Evaluation and performance standards for arterial prostheses. J Vasc Surgery, 1993, 17:746-56.

2) Greenwald SE and Berry CL. Improving vascular grafts: the importance of mechanical and haemodynamic properties. Journal of Pathology 2000; 190: 292-299.

3) Nerem RM, Seliktar D. Vascular Tissue Engineering. Annual Review of Biomedical Engineering. 2001; 3: 225-243.

4) Ariyoshi H., Okuyama M., Okahara K., et al. Expanded polytetrafluoroethylene (ePTFE) vascular graft loses its thrombogenicity six months after implantation. Thromb Res 1997; 88 (5): 427-33.

5) Poole-Warren L.A., Schindhelm K., Graham A.R., et al. Performance of small diameter synthetic vascular prosthesis with confluent autologos endothelial cell linings. J Biomed Mater Res 1996; 30 (2): 221-29.

6) Garrett HE, Dennis EW, DeBakey ME. Aortocoronary bypass with saphenous vein graft: seven year follow-up. JAMA 1973; 223: 792–794.

7) Boyer M., Townsend L.E., Vogel L.M., et al. Isolation of endothelial cells and their progenitor cells from human peripheral blood. J Vasc Surg 2000 Jan; 31(1 Pt 1):181-9.

8) L'Heureux N, Paquet S, Labbe R, et al. A completely biological tissue-engineered human blood vessel. FASEB J. 1998, 12(1):47-56.

9) Niklason LE. Techview: medical technology. Replacement arteries made to order. Science. 1999; 286(5444):1493-4.

10) Campoccia D, Doherty P, Radice M, Brun P, Abatangelo G, Williams DF: Semisynthetic resorbable materials from hyaluronan esterification. Biomaterials 1998, 19(23):2101-2127.

11) Caplan A.I. “Tissue engineering design for the future: new logics, old moledeles”. Tissue Engineering 2000, 6:1-8.

12) Muller-Glauser W., Zilla P., Lachat M., Bisang B., Riesre F., Von Segesser L., Turina M. Immediate shear stress of endothelial cell monolayers seeded in vitro on fibrin glue-coated e PTFE prosthesis. Eur J Vasc Surg 1993, 7:324-328.

13) Zisch AH, Schenk U, Schense JC, et al. Covalently conjugated VEGF--fibrin matrices for endothelialization. J Control Release. 2001,72(1-3):101-13.

14) Gehling U., Ergun S., Schumacher U., Wagener C., Pantel K., Otte M., Schuch G., Schafhausen P., Mende T., Kilic N., Kluge K., Schafer B., Hossfeld D., Fiedler W. In vitro differentiation of endothelial form AC133-positive progenitor cells. Blood 2000, 95 (10):3106-3112.

15) Matsuda T., Nakayama Y. Surface microarchitectural design in biomedical applications: in vitro transmural endothelialization on microporous segmented polyurethane films fabricated using an excimer laser. J Biomed Mater Res1996; 31 (2): 235-42.

16) Pereira RF, Halford KW, O'Hara MD, Leeper DB, Sokolov BP, Pollard MD, Bagasra O, Prockop DJ. Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice. Proc Natl Acad Sci U S A. 1995; 92(11): 4857-61.

17) Orlic D, Kajstura J, Chimenti S, Limana F, Jakoniuk I, Quaini F, Nadal-Ginard B, Bodine DM, Leri A, Anversa P. Mobilized bone marrow cells repair the infarcted heart, improving function and survival. Proc Natl Acad Sci U S A. 2001; 98(18): 10344-9.

18) Lagasse E, Connors H, Al-Dhalimy M, Reitsma M, Dohse M, Osborne L, Wang X, Finegold M, Weissman IL, Grompe M. Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. Nature Medicine 2000; 6(11): 1229-1234

19) Theise ND, Nimmakayalu M, Gardner R, Illei PB, Morgan G, Teperman L, Henegariu O, Krause DS. Liver from bone marrow in humans. Hepatology 2000; 32(1): 11-6.

20) Miwa H. and Matsuda T. An integrated approach to the design and engineering of hybrid arterial prostheses. J Vasc Surg 1994; 19: 658-67.

21) Shindo S., Takagi A., Whittemore AD. Improved patency of collagen-impregnated grafts after in vitro autogenous endothelial cell seeding. J Vasc Surg 1987; 6: 325-32.

22) Shimizu K, Sugiyama S, Aikawa M et al. Host bone –marrow cells are a source of donor intimal smooth-muscle-like cells in murine aortic transplant arteriopathy. Nature Medicine 2001; 7: 738-41.

23) Tassiopoulos AK, Greisler HP. Angiogenic mechanisms of endothelialization of cardiovascular implants: a review of recent investigative strategies. J Biomater Sci Polym Ed. 2000;11(11):1275-84.

24) Kaushal S, Amiel GE, Guleserian KJ, et al. Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo. Nat Med. 2001, 7(9):1035-40.

25) Kim BS, Nikolovski J, Bonadio J, Mooney DJ. Cyclic mechanical strain regulates the development of engineered smooth muscle tissue. Nat Biotechnol. 1999; 17(10): 979-83.

26) Sumpio BE, Banes AJ, Link WG, Johnson G Jr. Enhanced collagen production by smooth muscle cells during repetitive mechanical stretching. Arch Surg. 1988; 123(10): 1233-6.

27) Birukov KG, Shirinsky VP, Stepanova OV, Tkachuk VA, Hahn AW, Resink TJ, Smirnov VN. Stretch affects phenotype and proliferation of vascular smooth muscle cells. Mol Cell Biochem. 1995; 144(2): 131-9.

28) Kanda K, Matsuda T. In vitro reconstruction of hybrid arterial media with molecular and cellular orientations.Cell Transplant. 1994; 3(6): 537-45.

29) Seliktar D, Black RA, Vito RP, Nerem RM. Dynamic mechanical conditioning of collagen-gel blood vessel constructs induces remodeling in vitro. Ann Biomed Eng. 2000; 28(4): 351-62.

30) Niklason LE, Gao J, Abbott WM, Hirschi KK, Houser S, Marini R, Langer R. Functional arteries grown in vitro. Science. 1999; 284(5413): 489-93.

Parole Chiave

Ingegneria tissutale; Matrici polimeriche biodegradabili; Acido ialuronico; Matrici biologiche acellulari; Bioreattori; Cellule endoteliali progenitrici