RICERCA ITALIANA     

FIRB simili:

• 1 - Identificazione, purificazione, transdifferenziazione e banking di cellule staminali indirizzate verso fenotipi propri e non propri da utilizzare per riparazione tissutale
• 2 - ESPANSIONE E DIFFERENZIAZIONE IN VITRO DI CELLULE STAMINALI CORDONALI E DA LIQUIDO AMNIOTICO DA UTILIZZARE NELL'INGEGNERIA TESSUTALE
• 3 - www*.neuralstemcells.it (*where,when,why)
• 4 - L'ingegneria dei tessuti per la rigenerazione dei tessuti connettivi con particolare riferimento al tessuto osseo
• 5 - Il trapianto cellulare come alternativa al trapianto d'organo: studio dei processi di crescita e differenziamento delle cellule staminali orientate verso fenotipi cardiovascolari.
• 6 - Ottimizzazione dell' espansione ex-vivo di cellule staminali e progenitori emopoietici umani
• 7 - COMPARTIMENTALIZZAZIONE DELLA RISPOSTA IMMUNITARIA NELLA SCLEROSI MULTIPLA: IDENTIFICAZIONE DEI MECCANISMI PATOGENETICI COME BERSAGLIO DI UN INTERVENTO TERAPEUTICO MIRATO.
• 8 - Impiego delle tecniche di ingegneria tissutale nello sviluppo di protesi vascolari cellularizzate
• 9 - INGEGNERIA TISSUTALE: SVILUPPO DI NUOVE STRATEGIE PER LA RIGENERAZIONE DEL PARODONTO E DELL'OSSO ALVEOLARE MEDIANTE CELLULE MESENCHIMALI DI DERIVAZIONE EMATOPOIETICA NELL'ANIMALE E NELL'UOMO. VALUTAZIONE DEGLI EFFETTI SISTEMICI.
• 10 - Studio dell'identità cellulare e dei meccanismi di differenziazione cellulare nell'era della postegnomica utilizzando tre situazioni paradigmatiche: la mammella, l'osso e i linfociti

Classificazione scientifico-disciplinare

• Area scientifico disciplinare: Scienze mediche
  • PATOLOGIA GENERALE

Classificazione brevettuale

• CHEMISTRY; METALLURGY
  • BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF (biocides, pest repellants or attractants, or plant growth regulators, containing micro-organisms, viruses, microbial fungi, enzymes, fermentates or substances produced by or extracted from micro-organisms or animal material A01N63/00; food compositions A21, A23; medicinal preparations A61K; chemical aspects of, or use of materials for, bandages, dressings, absorbent pads or surgical articles A61L; fertilisers C05); PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS (preservation of living parts of humans or animals A01N1/02); MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (micro-biological testing media C12Q)

Classificazione geografica

• Regione: Lombardia

Bibliografia

Anderson DJ, Gage FH, Weissman IL.(2001) Can stem cells cross lineage boundaries?
Nat Med. 7:393-5.

Asahara, T., Murohara, T., Sullivan, A., Silver, M., van der Zee, R., Li, T., Witzenbichler, B., Schatteman, G., and Isner, J. M. (1997). Isolation of putative progenitor endothelial cells for angiogenesis. Science 275, 964-7.
Asahara, T., Masuda, H., Takahashi, T., Kalka, C., Pastore, C., Silver, M., Kearne, M., Magner, M., and Isner, J.M. (1999). Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization. Circ. Res. 85, 221-228.

Beauchamp, J.R., Morgan, J. E., Pagel C. N, Partridge T. A. (1999) Dynamics of myoblast transplantation reveal a discrete minority of precursors with stem cell-like properties as the myogenic source. J.Cell Biol. 144, 1113-1121.

Bhatia M., Wang J.C.Y., Kapp U., Bonnet D., Dick J.E. Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice. Proc. Natl. Acad. Sci. USA, 94, 5320-5325, 1997.
De Angelis, L., Berghella, L., Coletta, M., Cusella De Angelis, M.G., Lattanzi, L., Ponzetto C., and Cossu, G. (1999). Skeletal myogenic progenitors originating from embryonic dorsal aorta co-express endothelial and myogenic markers and contribute to post-natal muscle growth and regeneration. J. Cell Biol. 147, 869-878.

Bjornson CR, Rietze RL, Reynolds BA, Magli MC, Vescovi AL (1999) Turning brain into blood: a hematopoietic fate adopted by adult neural stem cells in vivo. Science 283: 534-537.

Blau HM, Brazelton TR, Weimann JM. (2001) The evolving concept of a stem cell: entity or function? Cell 105:829-41.
Brazelton, T.R., Rossi, F.V., Keshnet, G.I., and Blau, H.M. (2000). From marrow to brain :expression of neuronal phenotypes in adult mice. Science 290, 1775-1779.
Clarke, D.L., Johansson, C.B., Wilbertz, J., Veress, B., Nilsson, E., Karlstrom, H., Lendahl U., and Frisen, J. (2000). Generalized potential of adult neural stem cells. Science 288, 1660-1663.

De Ruiter, M.C., Poelmann, R.E., VanMunsteren, J.C., Mironov, V., Markwald, R.R., and Gittenberger-de Groot, A.C. (1997). Embryonic endothelial cells transdifferentiate into mesenchymal cells expressing smooth muscle actins in vivo and in vitro. Circ. Res. 80, 444-51.

Dunlop DJ, Pragnell IB 1994. Efficient isolation of human CD34 positive hemopoietic progenitor cells by immune panninga. Stem Cells. 12:114-124.

Ferrari, G., Cusella-De Angelis, G., Coletta, M., Paolucci, E., Stornaiuolo, A., Cossu, G., and Mavilio, F. (1998). Muscle regeneration by bone marrow-derived myogenic progenitors. Science 279, 1528-30.

Follenzi, A., Ailles, L.E., Bakovic, S., Geuna, M., and Naldini, L. (2000) Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences. Nat Genet. 25, 217-222.

Hao QL, Thiemann FT, Petersen D, Smogorzewska EM, Crooks GM. Extended long-term culture reveals a highly quiescent and primitive human hematopoietic progenitor population. Blood 88, 3306-3313, 1996.

Gage, F.H. (2000) Mammalian neural stem cells. Science 287, 1433-1438.
Galli, R., Borello, U., Gritti, A., Minasi, M.G., Bjornson, C., Coletta, M., Mora, M., De Angelis, M.G., Fiocco, R., Cossu, G., and Vescovi, A.L. (2000). Skeletal myogenic potential of human and mouse neural stem cells. Nat. Neurosci. 3, 986-991.

Gussoni E, Blau HM, Kunkel LM (1997) The fate of individual myoblasts after transplantation into muscles of DMD patients. Nat Med. Sep;3:970-7.
Jackson, K.A., Mi, T., and Godell, M.A. (1999). Hematopoietic potential of stem cells isolated from murine skeletal muscle. Proc. Natl. Acad. Sci. USA 96, 14482-14486.
Gussoni, E., Soneoka, Y., Strickland, C.D., Buzney, E.A., Khan, M.K., Flint, A.F., Kunkel, L.M., and Mulligan, R.C. (1999). Dystrophin expression in the mdx mouse restored by stem cell transplantation. Nature 401, 390-394.
Lagasse, E., Connors, H., Al-Dhalimy, M., Reitsma, M., Dohse, M., Osborne, L., Wang, X., Finegold, M., Weissman, I.L., and Grompe, M. (2000). Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. Nat. Med. 6, 1229-1234.

Larochelle A, Vormoor J, Hanenberg H, Wang JC, Bhatia M, Lapidot T, Moritz T, Murdoch B, Xiao XL, Kato I, Williams DA, Dick JE. Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: implications for gene therapy. Nat Med. 2, 1329-1337, 1996.

Lindahl, P., Johansson, B.R., Leveen, P., and Betsholtz, C. (1997). Pericyte loss and microaneurysm formation in PDGF-B-deficient mice. Science 277, 242-245.

Medvinsky, A., and Dzierzak, E. (1996). Definitive hematopoiesis is autonomously initiated by the AGM region. Cell 86, 897-906.
Mezey, E., Chandross, K.J., Harta, G., Maki, R.A., and McKercher, S.R.. (2000). Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow. Science 290, 1779-1782.

Odorico JS, Kaufman DS, Thomson JM (2001) Multilineage differentiation from human embryonic stem cell lines. Stem Cells. 19:193-204.

Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, Pickel J, McKay R, Nadal-Ginard B, Bodine DM, Leri A, Anversa P. Bone marrow cells regenerate infarcted myocardium. Nature 410, 701-705, 2001.

Paterson, B.E., Bowen, W.C., Patrene, K.D., Mars, W.M., Sullivan, A.K., Murase, N., Boggs, S.S., Greenberger, J.S., and Goff, J.P. (1999). Bone marrow as a potential source of hepatic oval cells. Science 284, 1168-1170.

Risau W. (1997). Mechanisms of angiogenesis Nature 386: 671-674

Shultz L.D., Schweitzer P.A., Christianson S.W., Gott B., Schweitzer I.B., Tennent B., McKenna S., Mobraaten L., Rajan T.V., Greiner D.L., Leiter E.H. Multiple defects in innate and adaptive immunologic function in NOD/LtSz-scid mice. J. Immunol. 154, 180-191, 1995.

Smith, A. (1998). Cell therapy: in search of multipotency. Curr. Biol. 5, 802-804.

Sutherland HJ, Lansdorp PM, Henkelman DH, Eaves AC, Eaves CJ. Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers. Proc Natl Acad Sci U S A 87, 3584-3588, 1990.

Takahashi,T., C.Kalka, H.Masuda, D.Chen, M.Silver, M.Keraney, M.Magner, J.M.Isner, and T.Asahara. (1999) Ischemia and cytokine- induced mobilization of bonemarrow-derived endothelial progenitor cells for neovascularization. Nature Medicine 5: 434-438,

Temple, S. and Alavarez-Buylla, A. (1999) Stem cells in the adult mammalian nervous system. Curr. Op. Neurobiol. 9, 135-141.

Van der Loo JC, Slieker WA, Kieboom D, Ploemacher RE. Identification of hematopoietic stem cell subsets on the basis of their primitiveness using antibody ER-MP12. Blood 85, 952-962, 1995.

Vescovi AL, Snyder EY. (1999) Establishment and properties of neural stem cell clones: plasticity in vitro and in vivo. Brain Pathol. 9:569-98

Vittet, D., Prandini, M.H., Berthier, R., Schweitzer, A., Martin Sisteron, H., Uzan, G., and Dejana, E. (1996) Embryonic stem cells differentiate in vitro to endothelial cells through successive maturation steps. Blood., 88: 3424-3431

Wang, J.C.Y., Doedens, M., Dick J.E. Primitive human hematopoietic cells are enriched in cord blood compared with adult bone marrow or mobilized peripheral blood as measured by the quantitative in vivo SCID-repopulating cell assay. Blood, 89, 3919-3924, 1997.

Watt FM, Hogan BL (2000) Out of Eden: stem cells and their niches. Science. 287:1427-30.

Yamashita, J., Itoh, H., Hirashima, M., Ogawa, M., Nishikawa, S., Yurugi, T., Naito, M., Nakao K., and Nishikawa S. (2000). Flk-1 positive cells derived embryonic stem cell from serve as vascular progenitors. Nature 408, 92-96.

Ziegler, B.L., Valtieri, M., Porada, G.A., De Maria, R., Muller, R., Masella, B., Gabbianelli, M., Casella, I., Pelosi, E., Bock, T., Zanjani, E.D. and C. Peschle. KDR receptor: a key marker defining hematopoietic stem cells. Science 285, 1553-1558, 1999.

Parole Chiave

rigenerazione tissutale; cellule staminali; malattie degenerative; geni regolatori; trans-determinazione