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INIZIO_TESTO_DA_INDICIZZARE

Fund for investing in fundamental research

italiano - inglese
Research Units
  • Universita' degli Studi di PADOVA
    Dip. SCIENZE BIOMEDICHE SPERIMENTALI , PADOVA (PD)
  • Universit¿ degli Studi di PADOVA
    CHIMICA BIOLOGICA , PADOVA (PD)
  • Universita' degli Studi di GENOVA
    Dip. MEDICINA SPERIMENTALE , GENOVA (GE)
  • Universita' degli Studi di BARI
    Dip. FARMACO BIOLOGICO , BARI (BA)
  • Universita' degli Studi di MILANO-BICOCCA
    Dip. BIOTECNOLOGIE E BIOSCIENZE , MILANO (MI)
  • Scuola Internazionale Superiore di Studi Avanzati di TRIESTE
    Dip. SETTORE BIOFISICA , TRIESTE (TS)
  • Libera Universita' "Vita Salute S.Raffaele" MILANO
    Dip. MEDICINA E CHIRURGIA , MILANO (MI)
  • Universita' degli Studi di FERRARA
    Dip. MEDICINA SPERIMENTALE E DIAGNOSTICA , FERRARA (FE)
Similar FIRB:
Scientific and education field classification
International Patent Classification
  • CHEMISTRY; METALLURGY
    • BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
      • MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
      • MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF (biocides, pest repellants or attractants, or plant growth regulators, containing micro-organisms, viruses, microbial fungi, enzymes, fermentates or substances produced by or extracted from micro-organisms or animal material A01N63/00; food compositions A21, A23; medicinal preparations A61K; chemical aspects of, or use of materials for, bandages, dressings, absorbent pads or surgical articles A61L; fertilisers C05); PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS (preservation of living parts of humans or animals A01N1/02); MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (micro-biological testing media C12Q)
Geographical classification
Bibliografia
References

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Keywords
calcium; second messengers; biotechnology; gene expression; bioinformatics; proteomics

The ubiquitous Ca2+ signalling cascade: from molecules to functions

Università degli Studi di Padova
Abstract
Changes in intracellular Ca2+ concentration appear to play a universal role in cell activation and numerous signal transduction mechanisms converge ultimately on a common pathway that involves alterations of cellular Ca2+ levels. In turn Ca2+ modulates a series of downstream enzymatic cascades that eventually cooperate in the regulation of cellular responses. Derangements in cell Ca2+ homeostasis are often the cause of important human diseases and represent one of the key triggering events leading to cell toxicity and death.
As described in detail in the next sections, the purpose of this application is to create a network of research groups that, in spite of their origin from different backgrounds and institutions, are all concerned with the study of the mechanisms regulating signalling via Ca2+ and/or with the processes that are downstream of the Ca2+ signal. All these groups are specifically interested in, and have already contributed significant technological developments in the various fields of the proposal. An important aspect of their participation, therefore, will be the accessibility to all groups of these new opportunities and the coordinate development of any further technological progress.
The project is divided in 5 workpakages and within each one several activities are presented. The first two workpakages are primarily concerned with the technological aspects of the whole project. The main objectives are: i) the development of new fluorescent>>>

Principal Investigator
TULLIO POZZAN, Universita' degli Studi di PADOVA
Research Goal
The overall objective of the program is that of gaining a better understanding of the Ca2+ signalling pathway through the development and use of novel methodological approaches. A unique feature of the present project is that, though the joint efforts of several groups two very different types of methodologies, i.e. high throughput, but destructive, DNA chips or mass spectroscopy, and analytical methods, single live cell imaging, will be integrated to achieve one specific goal. Through these two very different, yet complementary, approaches we hope to gain a deeper understanding of this vital signalling pathway, essential not only in physiology, but also in a number of pathological conditions.
1. Development of new tools to the study of second messenger level in living cells.
The unit coordinated by Dr. Pozzan will be concerned with the refinement of new probes (fluorescent and chemiluminescent) for investigating Ca2+ levels at the subcellular level. The research unit coordinated by Dr. Rizzuto will focus its attention on the development of new probes for the quantitative measurements of "ATP sensors" that will allow to measure ATP fluctuations in restricted compartments of the cytoplasm or changes in ATP concentration in the pericellular space (e.g. in response to cell stimulation by neurotransmitters or growth factors). The same groups will be concerned with the development of new probes to monitor quantitatively the activation of PKC isoforms>>>

Timescale
36 months