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INIZIO_TESTO_DA_INDICIZZARE

Fund for investing in fundamental research

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Research Units
  • Universita' degli Studi di ROMA "La Sapienza"
    Dip. GENETICA E BIOLOGIA MOLECOLARE , ROMA (RM)
  • Universita' degli Studi di MILANO-BICOCCA
    Dip. BIOTECNOLOGIE E BIOSCIENZE , MILANO (MI)
  • Universita' degli Studi di ROMA "La Sapienza"
    Dip. SCIENZE BIOCHIMICHE , ROMA (RM)
  • Consiglio nazionale delle ricerche (CNR)
    Istituto di Biologia Cellulare , ROMA (RM)
  • Universita' degli Studi di PARMA
    Dip. BIOCHIMICA E BIOLOGIA MOLECOLARE , PARMA (PR)
  • International Centre for Genetic Engineering and Biotechnology
    ICGEB (International Centre for Genetic Engineering and Biotechnology) , TRIESTE (TS)
  • Universita' degli Studi di MILANO
    Dip. GENETICA E BIOLOGIA DEI MICRORGANISMI , MILANO (MI)
  • Universita' degli Studi di ROMA "La Sapienza"
    Dip. BIOLOGIA CELLULARE E DELLO SVILUPPO , ROMA (RM)
Similar FIRB:
Scientific and education field classification
International Patent Classification
  • CHEMISTRY; METALLURGY
    • BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
      • MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF (biocides, pest repellants or attractants, or plant growth regulators, containing micro-organisms, viruses, microbial fungi, enzymes, fermentates or substances produced by or extracted from micro-organisms or animal material A01N63/00; food compositions A21, A23; medicinal preparations A61K; chemical aspects of, or use of materials for, bandages, dressings, absorbent pads or surgical articles A61L; fertilisers C05); PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS (preservation of living parts of humans or animals A01N1/02); MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (micro-biological testing media C12Q)
Geographical classification
Bibliografia
1) Warner JR. Trends Biochem Sci. 1999 24:437-40
2) Bordi L, Cioci F, Camilloni G. Mol Biol Cell. 2001 12:753-60.
3) Vega-Palas MA, Venditti S, Di Mauro E. Nat Genet. 1997 15:232-3.
4) Li B, Oestreich S, de Lange T. Cell 2000 101:471-83
5) Wyrick JJ, Holstege FC, Jennings EG, Causton HC, Shore D, Grunstein M, Lander ES, Young RA Nature 1999 402:418-21.
6) Reid JL, Iyer VR, Brown PO, Struhl K. Mol Cell. 2000 6:1297-307.
7) Iyer VR, Horak CE, Scafe CS, Botstein D, Snyder M, Brown PO. Nature 2000 409:533-8.
8) Venditti S, Di Stefano G, Di Mauro E. Telomere-based neo-Darwinian selection of yeast clonal subpopulations. Mol Gen Genet. 2000 263:787-95
9) Lieb JD, Liu X, Botstein D, Brown PO Nat. Genet. 2001 28:327-34.
10) Fiorani P, Amatruda JF, Silvestri A, Butler RH, Bjornsti MA, Benedetti P. Mol Pharmacol. 1999; 56:1105-15.
11) S. Venditti , G. Di Stefano and E. Di Mauro Food Technology And Biotechnology 2000 38, 1-8
12) S. Venditti, M. A. Vega-Palas, G. Di Stefano and E. Di Mauro Mol.Gen. Genet. 1999, 262, 367-377
13) S. Venditti, M. A. Vega-Palas and E. Di Mauro J. Biol. Chem. 1999, 274, 1928-1933
14) M. A. Vega-Palas, S. Venditti and E. Di Mauro J. Biol. Chem., 1998, 273, 9388-9392
15) V. De Sanctis, C. Bertozzi, G. Costanzo, E.Di Mauro and R. Negri Radiation Research , 156, 2675-2683 (2001)
16) R. Negri, M. Buttinelli, G. Panetta, V. De Arcangelis, E. Di Mauro and A. Travers J. Mol. Biol. 307, 987-1000 (2001)
17) R. Negri, M. Buttinelli, G. Panetta, V. De Arcangelis, E. Di Mauro and A. Travers Biochemical Society Transactions (2000) 28, 373-376
18) E. Di Mauro, S.G. Kendrew and M. Caserta J.Biol. Chem. (2000) 275, 7612-7618
19) L. Di Marcotullio, M. Buttinelli, G. Costanzo, E. Di Mauro and R. Negri Biochem. J., 333, 65-69 (1998)
20) L. Verdone, F. Cesari, C.L. Denis,E. Di Mauro and M. Caserta The J.Biol. Chem., 272, 30828-30834 (1997)
21) M. Caserta and E. Di Mauro BioEssays, 18, 947-955 (1996)
22) L. Verdone, G. Camilloni, E. Dimauro and M. Caserta Mol. Cell. Biol. 16, 1978-1988 (1996)
23) M. Buttinelli, R. Negri, L. Di Marcotullio and E. Di Mauro Proc.Natl Acad Sci U.S.A. 92, 10747-10751 (1995)
24) G. Costanzo, E. Di Mauro, R. Negri, G. Pereira and C. Hollenberg The J. of Biol. Chem. 270, 11091-11097 (1995)
25) L. Rubbi, G. Camilloni, M. Caserta, E. Di Mauro and S. Venditti Biochem. J. 328, 401-407 (1997)
26) R.K.Z. Tan, S.C.Harvey, E.Di Mauro, G. Camilloni and P. Venditti J. of Biomolecular Structure and Dynamics, 13, 855-872 (1996)
27) M. Vogelauer, F. Cioci and G. Camilloni J. Mol. Biol. 275, 197-209 (1998)
28) G. Camilloni, R. Negri, M, Caserta and E. Di Mauro In Methods In Mol. Biology, Protocols for DNA Topoisomerases. Vol. 54, 51-60 (1999)
29) M. Vogelauer and G. Camilloni J. Mol. Biol. 293, 19-28 (1999)
30) Nielsen, P. E. 1999. Applications of peptide nucleic acids. Curr. Opin. Biotechnol. 10:71-75.
31) Braguglia D., P. Heun, P. Pasero, B. Duncker and S. Gasser. 1998. J. Mol. Biol. 281:631.
32) Deshpande A. and C. Newlon. 1996. Science 272:1030
33) Dieci G., R. Corradini, S. Sforza, R. Marchelli and S. Ottonello. 2001. J. Biol. Chem. 276:5720.
34) Dieci G., L. Duimio, F. Coda, K. Sprague and S. Ottonello. 1993. J. Biol. Chem. 268:11199.
35) Dieci G., S. Hermann-Le Denmat, E. Lukhtanov, P. Thuriaux, M. Werner and A. Sentenac. 1995. EMBO J. 14:3766.
36) Donze D. and R. Kamakaka. 2001. EMBO J. 20:520.
37) Hull M., J. Erickson, M. Johnston and D. Engelke. 1994. Mol. Cell. Biol. 14:1266.
38) Ottonello S., D. Rivier, G. Doolittle, L. Young and K. Sprague. 1987. EMBO J. 6:1921.
39) Percudani R. 2001. Trends Genet. 17:133.
40) Percudani R. and S. Ottonello. 1999. Mol. Biol. Evol. 16:1752.
41) Percudani R., A. Pavesi and S. Ottonello. 1997J. Mol. Biol. 268:322.
42) Rivetti C., M. Guthold and C. Bustamante. 1999EMBO J. 18:4464.
43) Rüth J., C. Conesa, G. Dieci, O. Lefebvre, A. Düsterhoft, S. Ottonello and A. Sentenac. 1996. EMBO J. 15:1941.
44) Sforza S., R. Corradini, S. Ghirardi, A. Dossena and R. Marchelli. 2000. Eur. J. Org. Chem. :2905.
45) Teichmann M., G. Dieci, J. Huet, J. Ruth, A. Sentenac and K. Seifart. 1997. EMBO J 16:4708.
46a) Dieci, G., and A. Sentenac. 1996. Cell 84:245-252.
46b) Lopes M. Cotta-Ramusino C. Pellicioli A., Liberi G., Plevani P., Muzi-Falconi M., Newlon C.S., Foiani M. (2001). Nature. 412, 557-561
47) Foiani, M., Pellicioli,A., Lopes,M., Lucca,C., Ferrari,M., Liberi,G.,;Muzi Falconi,M., Plevani P. (2000). Mutation Research. 451,187-196
48) Liberi,G., Chiolo,I., Pellicioli,A., Lopes, M., Plevani P. Muzi Falconi, M., Foiani,M. (2000). Embo J.. 19: 5027-5038
49) Pellicioli, A. Lucca, C. Liberi, G. Marini, F. Lopes,M. Plevani P. Romano, A. Di Fiore, P.P. Foiani, M. (1999). Embo J.. 18:. 6561-6572
50) Longhese, M.P. Foiani, M. Muzi Falconi, M. Lucchini, G., Plevani P. (1998) Embo J. 17:5525-5528
51) Hartwell L.H. and Kastan M.B. (1994) Science 266: 1821-1827
52) Zhou B.B. and Elledge S.J. (2000) Nature 408: 433-439
53) Halazonetis T.D. and Shiloh Y. (1999) Biochim. Biophy. Acta 1424: 45-55
54) Wassmann K., and Benezra R. (2001) Curr. Opin. Genet. Dev. 11, 83-90
55) Hoyt, M.A. (2000) Cell 102, 267-270
56) O’Connel M.J., Walworth N.C. and Carr A.M. (2000) Trends Cell Biol. 10: 296-303
57) Lowndes N.F. and Murgia J.R. (2000) Curr. Opin. Genet. Dev. 10: 17-25
58) Paciotti V., Clerici M., Lucchini G. and Longhese M.P. (2000) Genes & Dev. 14: 2046-2059
59) Hwang L.H. et.al.(1998) Science 279, 1041-1044
60) Zachariae W. and Nasmyth K. (1999) Genes Dev 13, 2039-2058
61) Shirayama M., Toth A., Galova M. and Nasmyth K. (1999) Nature 402, 203-207
62) Visintin R., Craig K., Hwang E.S., Prinz S.,Tyers M. and Amon, A. (1998) Mol. Cell 2, 709-718
63) Diffley J.F. (2001) Curr. Biol.11, 367-370
64) Bardin A.J., Visintin R., and Amon A. (2000) Cell 102, 21-31
65) Bloecher A., Venturi G.M., and Tatchell K. (2000) Nat Cell Biol 2, 556-558
66) Pereira G., Hoefken T., Grindlay J., Manson C., and Schiebel E. (2000) Mol Cell 6, 1-10
67) Elledge, S.J. Science, 1996, 274, 1664
68) Abdurashidova et al. 1998, EMBO J. 17, 2961-2969
69) Abdurashidova et al. 2000, Science 287, 2023-2026
70) King R.W., Deshaies R.J., Peters J-M. and Krschner M-W. 1996. Science 274, 1652-1658
71) Kornitzer D., Raboy B., Kulka R.C., Fink G.R. 1994. EMBO J 13, 6021-6030
72) Stitzel M.L., Durso R. and Reese J.C. 2001 Genes Dev. 15, 128-133
73) Lam Y.A. et al. 2000. Proc Natl Acad Sci USA, 97, 9902-9906
74)Gazos Lopes U., Erhardt P., Yao R. and Cooper G.M. 1997. J Biol. Chem. 272, 12893-12896
75) Glickman M.H., Rubin D.M., Coux O., Wefes I., Pfeifer G., Cjeka Z., Baumeister W., Fried V.A. and Finley D. 1998. Cell 94, 615-623
76) Rinaldi T., Ricci C., Porro D., Bolotin-Fukuhara M. and Frontali L. 1998 Mol. Biol. Cell 9, 2917-2931
77) De Santis, V., Bertozzi, C., Costanzo, G., Di Mauro, E. and Negri R. 2001. Radiation Research 156, 379-387
78) Seifert, R., Wenzel-Seifert, K. and Kobilka, B.K. Trends Pharmacol. Sci. 20:383-389, 1999.
79) Milligan, G.. Trends Pharmacol. Sci. 21:24-28, 2000.
80) Wurch, T. and. Pauwels P. J.. J. of Pharmacological and Toxicological Methods, 45: 3-16, 2001.
81) Medici, R., Bianchi, E., Di Segni, G. and Tocchini-Valentini, G.P. EMBO J. 16: 7241-7249, 1997.
82) Benedetti, P., P. Fiorani, L. Capuani, and J. C. Wang. Cancer Research 53:4343-4348 (1993).
83) Ostrander, E.O., Benedetti, P. and Wang, J.C. Science (1990) 249: 1261-1265 IF 21.911
84)Benedetti, P., Fiorani, P., Capuani, L., Wang, J. C. (1993). Canc. Res. 53: 4343-4348
85) Benedetti, P., Silvestri,A., Fiorani, P. and Wang, J.C 1997 JBC 272 12132-12137
86) Hann, C., Evans,D.L., Fertala,J., Benedetti,P., Bjornsti,M.-A. and Hall,D.J. J. Biol. Chem. 273:8425-8433. (1998)
Keywords
post genomics; proteomics; complex cellular functions; cell cycle; transcription regulators; structural biology

Genomics And Proteomics in the analysis of complex cellular functions.(GAP)

Università degli Studi di Roma "La Sapienza"
Abstract
Making sense of the DNA sequence information is a biological issue.
A wealth of data describing the linear sequence of chromosomal DNA from multiple organisms is now available, their systematization is a complex task. We propose an integrated set of approaches in order to fill this GAP, facing the various facets of the post-genomic logics and to develop the necessary technologies. In a pure post-genomic perspective we will perform
(a) whole-genome studies, namely :
- whole-genome analyses of gene expression in RNA polymerase I, II and III-served large groups of genes,
- whole-genome analyses of protein patterns,
- whole-genome analyses of the effects of post-translational modifications of proteins.
(b) Studies focused on the determination of protein structure and protein-protein interactions will be performed on macromolecules in the broad-range model systems studied. These analyses will be relevant per se and in the discovery of general rules through comparative studies.
The Research Team is composed of 8 Units: RU # 1 DI MAURO,RU # 2 OTTONELLO, RU # 3 VINDIGNI, RU # 4 PLEVANI, RU # 5 LUCCHINI, RU # 6 FRONTALI, RU # 7 DI SEGNI, RU # 8 TSERNOGLOU
The scheme of the problems to be faced is:
- definition of the protein patterns controlled by broad-range regulatory networks. Namely: (i) the protein patterns under the control of the Regulatory Activator Protein 1 (Rap1) which controls 294>>>

Principal Investigator
ERNESTO DI MAURO, Universita' degli Studi di ROMA "La Sapienza"
Research Goal
The full understanding of the wealth of data obtained by the determination of entire genomes and produced by the analyses currently performed on genome-wide expression patterns requires the determination of (i) the functional integrations among genes and of (ii) the interactions among their products. We have planned a coherent ensemble of approaches and of integrated model systems in order to contribute to fill this GAP.
We will focus on the major groups of cellular functions: 1) WIDE-RANGE TRANSCRIPTION FRAMES, 2) GENETIC PATHWAYS AND FUNCTIONS INVOLVED IN DNA REPLICATION, REPAIR, SEGREGATION, AND IN CELL CYCLE CONTROL, 3) SIGNAL TRANSDUCTION SYSTEMS, mostly related to the connections between transcription regulations and cell cycle controls, 4) the FUNCTION OF THE PROTEASOME, a multiprotein quasi-organelle, in protein flows.
The approaches to be used rely on both highly advances genetic knowledge and capabilities, and on up-front technologies (i.e.: genome-wide microarrays of gene expression, genome-wide analyses of protein expression and modification patterns, innovative microarrays based analytical approaches)(See Sect. 3.3.). Several of the proteins endowed of central broad-range regulatory and activator functions with key roles in the systems considered will be the focus of STRUCTURAL STUDIES to be performed with advanced technologies (mass spec., X-ray crystallography, others: see Section 3.2). The overall approach will be characterized by a double>>>

Timescale
36 months