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INIZIO_TESTO_DA_INDICIZZARE

Fund for investing in fundamental research

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Research Units
  • Universita' degli Studi di PAVIA
    Dip. BIOCHIMICA , PAVIA (PV)
  • Universita' degli Studi di PAVIA
    Dip. MEDICINA SPERIMENTALE , PAVIA (PV)
  • Universita' degli Studi di TORINO
    Dip. GENETICA, BIOLOGIA E BIOCHIMICA , TORINO (TO)
  • Istituto di Ricerche Farmacologiche Mario Negri
    Dipartimento di Immunologia e Biologia Cellulare , MILANO (MI)
  • Universita' degli Studi di PAVIA
    Dip. GENETICA E MICROBIOLOGIA 'A.BUZZATI-TRAVERSO , PAVIA (PV)
  • Universita' degli Studi di PAVIA
    Dip. GENETICA E MICROBIOLOGIA 'A.BUZZATI-TRAVERSO , PAVIA (PV)
  • Universita' degli Studi di GENOVA
    Dip. MEDICINA SPERIMENTALE , GENOVA (GE)
  • Universita' degli Studi di URBINO
    Ist. Chimica biologica , PESARO (PU)
  • IRCCS POLICLINICO SAN MATTEO, PAVIA
    Clinica Medica III , PAVIA (PV)
Similar FIRB:
Scientific and education field classification
International Patent Classification
  • CHEMISTRY; METALLURGY
    • BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
      • MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF (biocides, pest repellants or attractants, or plant growth regulators, containing micro-organisms, viruses, microbial fungi, enzymes, fermentates or substances produced by or extracted from micro-organisms or animal material A01N63/00; food compositions A21, A23; medicinal preparations A61K; chemical aspects of, or use of materials for, bandages, dressings, absorbent pads or surgical articles A61L; fertilisers C05); PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS (preservation of living parts of humans or animals A01N1/02); MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (micro-biological testing media C12Q)
Geographical classification
Bibliografia
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22. G.H. Van Zanten, P.G. De Groot, J.S. Sixma "Platelet adhesion" (1997) in Handbook of Experimental Pharmacology, vol. 126: "Platelets and their factors", pag. 61- 81, von Bruchhausen F. and Walter U. eds., Springer-Verlag, Berlin
23. K.J. Clemetson, J. Polgar "Platelet adhesion and aggregation receptors" (1997) in Handbook of Experimental Pharmacology, vol. 126: "Platelets and their factors", pag. 155 - 179, von Bruchhausen F. and Walter U. eds., Springer-Verlag, Berlin
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29. Sturla L, Puglielli L, Tonetti M, Berninsone P, Hirschberg CB, De Flora A, Etzioni A. (2001) Pediat Res. 49: 537-542
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31. Rizzi, M., Tonetti, M., Vigevani, P., Sturla, L., Bisso, L., De Flora, A., Bordo, D., and Bolognesi, M. (1998) Structure 6: 1453-1465
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Keywords
GROWTH FACTORS; ANGIOGENESIS; ADHESION; SIGNAL TRANSDUCTION; PROTEIN STRUCTURE; PROTEOLYSIS

Molecular recognition and cell function

Università degli Studi di Pavia
Abstract
The present project focuses its attention on the mechanisms by which the interactions among molecules able to recognise themselves in a specific way influence cellular functionality. The project will be carried out not only by studying the cellular effects of the interaction between wild type molecules, but also by investigating the consequences of the inhibition of these specific interactions and the consequences of the modifications obtained by mutagenesis of the involved proteins and their molecular partners. The project is therefore focused on establishing the exact correlation between the structure of interacting molecules, the type of the interaction and the consequence of such interaction on cellular functionality.
The project is divided in four workpackages, each of them regarding specific topics in cellular physiology.
The first WP will use as an experimental model the study of the interactions between growth factors (GF) and their receptors. It will be focused on the possibility to modify specific domain of GF by genetic engineering in order to obtain useful tools for therapeutic purposes. Moreover, the project will investigate the role of semaphorine, a protein involved in angiogenesis that is synthesised by GF-activated endothelial cells.
The second WP will focus its attention on the adhesive receptors binding to soluble or cell surface bound ligands. The attention will be placed on the mutual influences between the adhesion mediated by>>>

Principal Investigator
CESARE BALDUINI, Universita' degli Studi di PAVIA
Research Goal
The general aim of this project is to point out different experimental models to study the cellular functions as a consequence of a specific molecular recognition. Sometimes the interactions involve only two components, while, more frequently, they require several molecular structures to achieve the correct cellular function.
The proponent group therefore non only will investigate cellular events consequent to the interaction between two specific molecular components; it will also consider the structural characteristics of the interactions and their correlations with cell function consequences. Moreover it will be investigated the mechanisms by which the primary interaction and the cellular function are modified and regulated by the binding of other molecular components.
These projects are supported by the deep experience of the proponent groups in different fields of biological research that fit with the general topic of the present proposal. This experience results also in the availability in the different research units of advanced experimental model like mutant cell lines, transgenic mice and monoclonal antibodies.
On the basis of the above considerations the project considers four fields of biological research, very important for basic science but also strongly correlated with the pathogenesis of different diseases and with the development of diagnostic and therapeutic possibilities. Each of these topics represents an aim of the project and will>>>

Timescale
36 months