RICERCA ITALIANA     

Programmi di ricerca simili:

• 1 - Supramolecular complexes of sorcin in the generation and regulation of Calcium-dependent cellular functions
• 2 - Proprieta’ strutturali e attivita’ funzionale di un complesso proteico modificante la cromatina nell’uomo
• 3 - Analisi dell'interazione tra la proteina del nucleoide batterico H-NS e il DNA: caratterizzazione molecolare dei siti di nucleazione.
• 4 - Basi funzionali e aspetti strutturali del dominio extracellulare del beta-distroglicano, una proteina "natively unfolded", coinvolta in patologie neuromuscolari.
• 5 - Ruolo dell'interazione dei metalli con il sistema Ubiquitina/Proteasoma nella patogenesi delle malattie conformazionali
• 6 - La rete di Interazioni fra proteine coniugate a Nedd8 o mono-ubiquitina ed i loro recettori
• 7 - Proteine di regolazione nelle piante: analisi biomolecolare dell'interazione di proteine 14-3-3 e calmodulina con proteine bersaglio.
• 8 - Struttura e biogenesi di proteine di trasporto dei mitocondri
• 9 - Studi strutturali su proteine che legano molecole idrofobiche
• 10 - Interattomi proteici: identificazione e caratterizzazione di network cellulari in differenti condizioni fisiopatologiche

Classificazione scientifico-disciplinare

• Area scientifico disciplinare: Scienze biologiche
  • BIOCHIMICA
  • BIOLOGIA MOLECOLARE

Classificazione brevettuale

• CHEMISTRY; METALLURGY
  • BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF (biocides, pest repellants or attractants, or plant growth regulators, containing micro-organisms, viruses, microbial fungi, enzymes, fermentates or substances produced by or extracted from micro-organisms or animal material A01N63/00; food compositions A21, A23; medicinal preparations A61K; chemical aspects of, or use of materials for, bandages, dressings, absorbent pads or surgical articles A61L; fertilisers C05); PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS (preservation of living parts of humans or animals A01N1/02); MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (micro-biological testing media C12Q)
  • ORGANIC CHEMISTRY (such compounds as the oxides, sulfides, or oxysulfides of carbon, cyanogen, phosgene, hydrocyanic acid or salts thereof C01; products obtained from layered base-exchange silicates by ion-exchange with organic compounds such as ammonium, phosphonium or sulfonium compounds or by intercalation of organic compounds C01B33/44; macromolecular compounds C08; dyes C09; fermentation products C12; fermentation or enzyme-using processes to synthesise a desired chemical compound or composition or to separate optical isomers from a racemic mixture C12P; production of organic compounds by electrolysis or electrophoresis C25B3/00, C25B7/00)
    • PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

Classificazione geografica

• Regione: Lazio

Bibliografia

1. Grant R.A., Filman D.J., Finkel S.E., Kolter R. and Hogle J.M. (1998) “The crystal structure of Dps, a ferritin homolog that binds and protects DNA” Nat Struct Biol. 4, 294-303.
2. Ceci P., Cellai S., Falvo E., Rivetti G., Rossi G.L. and Chiancone E. (2004) “DNA condensation and self-aggregation of Escherichia coli Dps are coupled phenomena related to the properties of the N-terminus” Nucleic Acids Res. 32 (19), 5935-5944.
3. Ilari, A., Stefanini, S., Chiancone, E. and Tsernoglou, D. (2000) “The dodecameric ferritin from Listeria innocua contains a novel intersubunit iron-binding site” Nat. Struct. Biol. 7, 38-43.
4. Ceci P., Ilari A., Falvo E. and Chiancone E. (2003) “The Dps Protein of Agrobacterium tumefaciens Does Not Bind to DNA but Protects It toward Oxidative Cleavage” J. Biol. Chem. 278, 20319-20326.
5 Zhao G., Ceci P., Ilari A., Giangiacomo L., Laue T. M., Chiancone E. and Chasteen N. D. (2002) “Iron and Hydrogen Peroxide Detoxification Properties of DNA-binding Protein from Starved Cells” J. Biol. Chem. 277, 27689-27696.
6. Yang X., Chiancone E., Stefanini S., Ilari A. and Chasteen N.D. (2000) “Iron oxidation and hydrolysis reactions of a novel ferritin from Listeria innocua” Biochem J. 349, 783-786.
7. Papinutto E., Dundon W.G., Pitulis N., Battistutta R., Montecucco C. and Zanotti G. (2002) “Structure of two iron-binding proteins from Bacillus anthracis” J. Biol. Chem. 277, 15093-15098.
8. Tonello F., Dundon W.G., Satin B., Molinari M., Tognon G., Grandi G., Del Giudice G., Rappuoli R. and Montecucco C. (1999) “The Helicobacter pilori neutrophil-activating protein is an iron-binding protein with dodecameric structure” Mol. Microbiol. 34, 238-246.
9. Kauko A., Haataja S., Pulliainen A.T., Finne J. and Papageorgiou A.C. (2004) “Crystal structure of Streptococcus suis Dps-like peroxide resistance protein Dpr: implications for iron incorporation” J Mol Biol. 338 (3), 547-58.
10. Ueshima J., Shoji M., Ratnayake D.B., Abe K., Yoshida S., Yamamoto K. and Nakayama K. (2003) “Purification, gene cloning, gene expression, and mutants of Dps from the obligate anaerobe Porphyromonas gingivalis” Infect Immun. 71 (3), 1170-1178.
11. Meyers M.B., Zamparelli C., Verzili D., Dicker A.P., Blanck T.J.J. and Chiancone E. (1995) “Calcium-dependent translocation of sorcin to membranes: functional relevance in contractile tissue” FEBS Lett. 357, 230-234.
12. Ilari A., Johnson K.A., Nastopoulos V., Zamparelli C., Colotti G., Tsernoglou D. and Chiancone E. (2002) “The crystal structure of the sorcin calcium binding domain provides a model of Ca2+-dependent processes in the full-length protein” J. Mol. Biol. 317, 447-458.
13. Mella, M., Colotti, G., Zamparelli, C., Verzili, D., Ilari, A. and Chiancone, E. (2003) “Information transfer in the penta-EF-hand protein sorcin does not operate via the canonical structural/functional pairing. A study with site-specific mutants” J. Biol. Chem. 278, 24921-24928.
14. Brownawell A.M. and Creutz C.E. (1997) “Calcium-dependent binding of sorcin to the N-terminal domain of synexin (annexin VII)” J. Biol. Chem. 272, 22182-22190.
15. Meyers M.B., Pickel V.M., Sheu S., Sharma V.K., Scotto K.W. and Fishman G.I. (1995) “Association of sorcin with the cardiac ryanodine receptor” J. Biol. Chem. 270, 26411-26418.
16. Meyers M.B., Puri T.S., Chien A.J., Gao T., Hsu P.H., Hosey M.M. and Fishman G.I. (1998) “Sorcin associates with the pore-forming subunit of voltage-dependent L-type Ca2+ channels” J. Biol. Chem. 273, 18930-18935.
17. Seidler T., Miller S.L.W., Loughrey C.M., Kania A., Burow A, Kettlewell S., Teucher N., Wagner S., Kogler H., Meyers M.B., Hasenfuss, G. and Smith, G.L. (2003) “Effects of adenovirus-mediated sorcin overexpression on excitation-contraction coupling in isolated rabbit cardiomyocytes” Circ. Res. 93, 132-139.
18. Matsumoto T., Hisamatsu Y., Ohkusa T., Inoue N., Sato T., Suzuki S., Ikeda Y and Matsuzaki M. (2005) “Sorcin interacts with sarcoplasmic reticulum Ca(2+)-ATPase and modulates excitation-contraction coupling in the heart” Basic. Res. Cardiol. 100, 1-13.
19. Frenkiel-Krispin D., Ben-Avraham I., Englander J., Shimoni E., Wolf S.G. and Minsky A. (2004) “Nucleoid restructuring in stationary-state bacteria” Mol Microbiol. 51 (2), 395-405.
20. Ren B., Tibbelin G., Kajino T., Asami O., Ladenstein R. (2003) “The multi-layered structure of Dps with a novel di-nuclear ferroxidase center” J Mol Biol. 329 (3), 467-477.
21. Mohiddin S., Antaramiam, A., EF, F., Gomez, A. M., Lin, J.-P., Yu, Z.-X., Valdivia, H. H., and Fananapazir, L. (2002) Circulation 106, II-319.
22. Cheng H., Lederer W.J. and Cannell M.B. (1993) “Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle” Science 262, 740–744.
23. Lokuta A.J., Meyers M.B., Sander P.R., Fishman G.I. and Valdivia H.H. (1997) “Modulation of cardiac ryanodine receptors by sorcin” J Biol Chem. 272, 25333–25338.
24. Farrell E.F., Antaramian A., Rueda A., Gomez A.M. and Valdivia H.H. (2003) “Sorcin Inhibits Calcium Release and Modulates Excitation-Contraction Coupling in the Heart” J. Biol. Chem. 278, 34660-34666.
25. Bers D.M. (2004) “Macromolecular complexes regulating cardiac ryanodine receptor function” J Mol Cell Cardiol. 37, 417-429.
26. Zamparelli, C., Ilari, A., Verzili, D., Giangiacomo, L., Colotti, G., Pascarella, S., and Chiancone, E. (2000) “Structure-function relationships in sorcin, a member of the penta EF-hand family. Interaction of sorcin fragments with the ryanodine receptor and an Escherichia coli model system” Biochemistry 39, 658–666
27. Janvier N.C. and Boyett M.R. (1996) “The role of Na-Ca exchange current in the cardiac action potential” Cardiovasc Res. 32, 69-84.
28. Bers D.M. (2002) “Cardiac excitation-contraction coupling” Nature 415, 198-205.
29. Convery M.K. and Hancox J.C. (1999) “Comparison of Na+-Ca2+ exchange current elicited from isolated rabbit ventricular myocytes by voltage ramp and step protocols” Pflugers Arch. Eur J. Physiol. 437, 944–954.
30. Smith G.P. and Petrenko V.A. (1997) “Phage Display” Chem. Rev. 97, 391-410.

Parole Chiave

RICONOSCIMENTO MACROMOLECOLARE; PROTEINE DPS; COMPLESSI PROTEINE DPS-DNA; ATTIVITÀ FERROSSIDASICA; PROTEINE PEF; SORCINA; SCAMBIATORE SODIO-CALCIO (NCX); RECETTORI PER LA RIANODINA; TRASDUZIONE DEL SEGNALE MEDIATA DAL CALCIO