RICERCA ITALIANA     

Programmi di ricerca simili:

• 1 - Correlazione tra modificazioni post-traduzionali di fattori di trascrizione e profilo globale di geni regolati: un approccio integrato per identificare nuove connessioni tra pathways che regolano proliferazione, risposte al danno, differenziamento ed oncogenesi.
• 2 - Studio del coinvolgimento dei fattori trascrizionali E2F nei processi cellulari, nello sviluppo e nella riproduzione
• 3 - CARATTERIZZAZIONE DELLE VIE DI TRASDUZIONE DEL SEGNALE RESPONSABILI DELLE RISPOSTE PRO- E ANTI-INFIAMMATORIE DEI GRANULOCITI POLIMORFONUCLEATI.
• 4 - Analisi delle mappe trascrizionali e delle variazioni di moduli funzionali nel corso del differenziamento mieloide umano normale
• 5 - Dalla cromatina ai fattori di trascrizione: studio del meccanismi di regolazione trascrizionale in piante di interesse agrario
• 6 - Ruolo, Meccanismi molecolari e nuovi interattori delle proteine Prep/Meis/Pbx nello sviluppo embrionale.
• 7 - Basi metaboliche e molecolari della sindrome di Down
• 8 - Ruolo dei microRNA nel differenziamento cellulare e nella tumorigenesi
• 9 - RETI GENETICO-MOLECOLARI NELLO SVILUPPO NEURALE: DAL DIFFERENZIAMENTO ALLA FUNZIONE
• 10 - Meccanismi della citochinesi: identificazione di nuovi geni coinvolti nel processo e nella sua regolazione

Classificazione scientifico-disciplinare

• Area scientifico disciplinare: Scienze biologiche
  • BIOLOGIA MOLECOLARE
  • BIOLOGIA APPLICATA
  • GENETICA

Classificazione brevettuale

• CHEMISTRY; METALLURGY
  • BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF (biocides, pest repellants or attractants, or plant growth regulators, containing micro-organisms, viruses, microbial fungi, enzymes, fermentates or substances produced by or extracted from micro-organisms or animal material A01N63/00; food compositions A21, A23; medicinal preparations A61K; chemical aspects of, or use of materials for, bandages, dressings, absorbent pads or surgical articles A61L; fertilisers C05); PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS (preservation of living parts of humans or animals A01N1/02); MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (micro-biological testing media C12Q)

Classificazione geografica

• Regione: Lombardia

Bibliografia

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7. Chi, S-W., Ayed, A. and Arrowsmith C.A. 1999. Solution structure of a conserved C-terminal domain of p73 with structural homology to the SAM domain. EMBO J. 18, 4438-4445.
8. Yang A., Schweitzer R., Sun D.Q., Kaghad M., Walker N., Bronson R.T., Tabin C., Sharpe A., Caput D., Crum C. and McKeon F. 1999. p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development. Nature 398, 714-718.
9. Mills A.A., Zheng B., Wang X-J., Vogel H., Roop D.R. and Bradley A. 1999. p63 is a homologue required for limb and epidermal morphogenesis. Nature 398, 708-713.
10. McGrath J.A., et al. 2001. Hay-Wells syndrome is caused by heterozygous missense mutations in the SAM domain of p63. Human Mol. Genetics 10, 1-9.
11. Brunner, H.G., Hamel, B.C. and van Bokhoven, HH.. 2002. p63 gene mutations and human developmental syndromes. Am. J. Med. Genet. 112, 284-290.
12. Pellegrini P., et al. 2001. p63 identifies keratinocyte stem cells. Proc. Acad. Sci. USA 98, 3156-3161.
13. Di Iorio, E., Barbaro, V., Ruzza, A., Ponzin, D., Pellegrini, G. and De Luca, M. 2005. Isoforms of {Delta}Np63 and the migration of ocular limbal cells in human corneal regeneration. Proc. Natl. Acad. Sci. USA 102, 9523-9528.
14. Pediconi N. et al. 2003. Differential regulation of E2F1 apoptotic target genes in response to DNA damage. Nat Cell. Biol. 5, 552-8.
15. Vossio, S et al. 2002. DN-p73 is activated after DNA damage in a p53 dependent manner to regulate p53-induced cell cycle arrest. Oncogene 21, 3796-3803.
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18. Testoni B., Borrelli S., Candi E., Tenedini E., Alotto D., Castagnoli C., Piccolo S., Tagliafico E., Ferrari S., Melino G. and Mantovani R. Identification of a p63 regulatory network in keratinocytes. Submitted.
19. Taylor, W.R. and Stark, G.R. 2001. Regulation of the G2/M transition by p53. Oncogene 20, 1803-1815.
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21. Testoni B. and Mantovani R. 2006. The p63-NF-Y G2/M promoters repressive switch is altered by AEC mutations. Nucleic Acids Res. 34, 928-938.
22. Yun J, Chae HD, Choy HE, Chung J, Yoo HS, Han MH, Shin DY 1999 p53 negatively regulates cdc2 transcription via the CCAAT-binding NF-Y transcription factor J. Biol. Chem. 274, 29677-82.
23. Badie C, Itzhaki JE, Sullivan MJ, Carpenter AJ, Porter AC. 2000 Repression of CDK1 and other genes with CDE and CHR promoter elements during DNA damage-induced G(2)/M arrest in human cells. Mol. Cell Biol. 20, 2358-66.
24. Manni I, et al.. 2001 NF-Y mediates the transcriptional inhibition of the cyclin B1, cyclin B2, and cdc25C promoters upon induced G2 arrest. J. Biol. Chem. 276, 5570-6.
25. Murphy M., Ahn J., Walker K.K., Hoffman W.H., Evans R.M., Levine A.J., George D.L. 1999. Transcriptional repression by wild-type p53 utilizes histone deacetylases mediated by interaction with mSin3a. Genes Dev. 13, 2490-501.
26. Hoffman W.H., Biade S., Zilfou J.T., Chen J., Murphy M. 2002 Transcriptional repression of the anti-apoptotic survivin gene by wild type p53. J Biol Chem. 277(5):3247-57.
27. Mantovani R. 1999. The molecular biology of the CCAAT-binding factor NF-Y. Gene 239, 15-27.
28. Romier, C., Cocchiarella, F., Mantovani, R. Moras, D. 2002. The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y. J. Biol. Chem. 278, 1336-1345.
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30. Lin T, Chao C, Saito S, Mazur SJ, Murphy ME, Appella E, Xu Y. 2005. p53 induces differentiation of mouse embryonic stem cells by suppressing Nanog expression. Nat. Cell Biol. 7, 165-71.
31. Monte M, Benetti R, Buscemi G, Sandy P, Del Sal G, Schneider C. 2003. The cell cycle-regulated protein human GTSE-1 controls DNA damage-induced apoptosis by affecting p53 function. J. Biol. Chem. 278, 30356-64.
32. Monte M, Benetti R, Collavin L, Marchionni L, Del Sal G, Schneider C. 2004. hGTSE-1 expression stimulates cytoplasmic localization of p53. J.Biol. Chem. 279, 11744-52.
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36. Miyazaki K, Ozaki T, Kato C, Hanamoto T, Fujita T, Irino S, Watanabe K, Nakagawa T, Nakagawara A. 2003. A novel HECT-type E3 ubiquitin ligase, NEDL2, stabilizes p73 and enhances its transcriptional activity. Biochem Biophys Res Commun. 308, 106-13.
37. Costanzo A et al. 2002. DNA Damage-Dependent Acetylation of p73 Dictates the Selective Activation of Apoptotic Target Genes. Mol Cell. 9, 175-86.
38. Strano S, et al. 2005. The transcriptional coactivator Yes-associated protein drives p73 gene-target specificity in response to DNA Damage. Mol Cell. 18, 447-59.
39. Testa, A., G. Donati, P. Yan, F. Romani, T. H. Huang, M. A. Vigano, and R. Mantovani. 2005. Chromatin immunoprecipitation (ChIP) on chip experiments uncover a widespread distribution of NF-Y binding CCAAT sites outside of core promoters. J. Biol. Chem. 280, 13606-15.
40. Viganò, M.A., et al. Identification of p63 targets in keratinocytes by ChIP on chip on CpG islands and promoters arrays. Submitted.
41. Dalla E., et al. 2005. Discovery of 342 putative new genes from the analysis of 5'-end-sequenced full-length-enriched cDNA human transcripts. Genomics. 85, 739-51.
42. Vaziri H., Dessain S.K., Ng Eaton E., Imai S.I., Frye R.A., Pandita T.K., Guarente L., Weinberg R.A. 2001. hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.Cell 107, 149-59.
43. Luo, J., Nikolaev, A.Y., Imai, S., Chen, D., Su, F., Shiloh, A., Guarente, L., Gu, W. 2001. Negative control of p53 by Sir2alpha promotes cell survival under stress. J.Cell Biol. 160, 1017-27.
44. Fontemaggi G., et al.. 2002. Identification of direct p73 target genes combining DNA microarray and chromatin immunoprecipitation analyses. J. Biol. Chem. 277(45):43359-68.
45. Pediconi N et al. 2003. Differential regulation of E2F1 apoptotic target genes in response to DNA damage. Nat Cell Biol. 5, 552-8.
46. Flores ER et al. (2002) p63 and p73 are required for p53-dependent apoptosis in response to DNA damage. Nature, 416, 560-564.
47. Zantoni M, Dalla E, Policriti A, Schneider C. Motif Discovery fixing mismatch positions”, 17th Annual Symposium on Combinatorial Pattern Matching, submitted.
48. Simpson AJ, Caballero OL, Jungbluth A, Chen YT, Old LJ. 2005. Cancer/testis antigens, gametogenesis and cancer. Nat Rev Cancer. 5, 615-25.
49. Monte M, Simonatto M, Peche L.Y, Bublik D.R, Gobessi S, Pierotti M.A, Rodolfo M and Schneider C. 2006. MAGE-A tumor antigens target p53 transactivation function through HDACs recruitment and confer resistance to chemotherapeutic agents. Proc.Natl.Acad.Sci (USA) in press.

Parole Chiave

P53, P63, P73, MODIFICAZIONI POST-TRADUZIONALI, IMMUNOPRECIPITAZIONE DELLA CROMATINA CHIP, GENI BERSAGLIO IN VIVO, CHIP ON CHIP